1673-159X

CN 51-1686/N

沈思蔚,姜丽,左澳腾,等. 接种嗜盐四联球菌和多变拟威克酵母对模拟减盐甜瓣子发酵特性的影响[J]. 西华大学学报(自然科学版),2024,43(4):17 − 23. doi: 10.12198/j.issn.1673-159X.5464
引用本文: 沈思蔚,姜丽,左澳腾,等. 接种嗜盐四联球菌和多变拟威克酵母对模拟减盐甜瓣子发酵特性的影响[J]. 西华大学学报(自然科学版),2024,43(4):17 − 23. doi: 10.12198/j.issn.1673-159X.5464
SHEN Siwei, JIANG Li, ZUO Aoteng, et al. Effect of Inoculation with Tetracococcus Halophilus and Wickerhamiella Versatilis on the Fermentation Characteristics of Simulated Salt-reduced Tianbanzi[J]. Journal of Xihua University(Natural Science Edition), 2024, 43(4): 17 − 23.. DOI: 10.12198/j.issn.1673-159X.5464
Citation: SHEN Siwei, JIANG Li, ZUO Aoteng, et al. Effect of Inoculation with Tetracococcus Halophilus and Wickerhamiella Versatilis on the Fermentation Characteristics of Simulated Salt-reduced Tianbanzi[J]. Journal of Xihua University(Natural Science Edition), 2024, 43(4): 17 − 23.. DOI: 10.12198/j.issn.1673-159X.5464

接种嗜盐四联球菌和多变拟威克酵母对模拟减盐甜瓣子发酵特性的影响

Effect of Inoculation with Tetracococcus Halophilus and Wickerhamiella Versatilis on the Fermentation Characteristics of Simulated Salt-reduced Tianbanzi

  • 摘要: 为提高减盐甜瓣子的风味品质,以嗜盐四联球菌(Tetragenococcus halophilus)和多变拟威克酵母(Wickerhamiella versatilis)作为强化发酵菌株,利用高效液相色谱仪、全自动氨基酸分析仪和气相色谱–质谱联用仪分析两菌株对甜瓣子模拟发酵培养基风味品质的影响。结果表明:不接种的空白对照组(CK)、单独接种嗜盐四联球菌组(T)、单独接种多变拟威克酵母组(W)和1∶1比例共同接种嗜盐四联球菌和多变拟威克酵母组(TW)的4组样品共鉴定出7种有机酸、16种氨基酸;与不接菌组(CK)相比,同时接种组(TW)中有机酸总含量提升112.61%,氨基酸总含量提升76.53%;4组样品共鉴定出36种挥发性风味物质,其中12种化合物被筛选为差异化合物(VIP > 1,p < 0.05);基于多元统计分析发现乙酸甲酯、异戊酸甲酯、苯甲醇、苯乙醛和2-甲基吡嗪等特征风味物质在TW组显著富集。本研究可为提高减盐甜瓣子品质提供一定参考。

     

    Abstract: To improve the flavor quality of salt-reduced Tianbanzi, Tetragenococcus halophilus and Wickerhamiella versatilis were selected as fermentation strains to explore their effect on the flavor quality of salt-reduced Tianbanzi by high performance liquid chromatography, fully automated amino acid analyzer and gas chromatography-mass spectrometry. The results show that 7 organic acids and 16 amino acids are identified in the four groups samples of blank control group without inoculation(CK), Tetragenococcus halophilus separately inoculated(T), Wickerhamiella versatilis group separately inoculated (W), and Tetragenococcus halophilus and Wickerhamiella versatilis group are inoculated together in a ratio of 1:1(TW). Compared with the non-inoculated group (CK), the total organic acid and amino acid content of the TW group are increased by 112.61% and 76.53%, respectively. In addition, 36 volatile flavor substances are identified in all samples, of which 12 compounds are screened as differential compounds distinguishing different samples (VIP > 1, p < 0.05). Based on multivariate statistical analysis, it is found that characteristic aroma compounds such as methyl acetate, methyl isovalerate, benzyl alcohol, phenylacetaldehyde and 2-methylpyrazine are significantly enriched in the TW group. These results can provide certain guidelines for the fortified fermentation strategies of brewing condiments.

     

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