Abstract: The extraction method of bamboo leaf flavonoids was studied by using light bamboo leaves which is from Sichuan as raw materials.The response surface methodology was adopted to optimize the ultrasonic extraction process of bamboo leaf flavonoids.The single factor experiment was uitilized to determine the level of ethanol concentration, extraction time and extraction power.The three-factor and three-level response surface analysis method was used to determine the optimal process conditions for extracting bamboo leaf flavonoids by ultrasonic technology.The optimal process conditions is that the ethanol concentration volume fraction is 70%, extraction power 190 kW and extraction time 30 mins.Under this condition, the theoretical extraction rate is 5.6% and the measured value is 5.46% which is very close to the theoretical prediction.The results provide a test basis for the pilot test and the production of bamboo leaf flavonoids.