Abstract: In order to broaden the resources of carotenoid producing microorganisms, pixian Douban was used as the isolation source to screen the carotenoid producing strain GH-1, and the strain was identified by morphological, physiological and biochemical analysis and 16S rRNA sequence analysis. On the basis of single factor experiment, response surface methodology was used to optimize the cultivation conditions for carotenoid production. The antioxidant capacity of carotenoids was analyzed by free radical scavenging method. The results show that strain GH-1 is Gordonia rubripertincta. The optimal culture conditions are as follows: incubation time 145 h, inoculation amount 8%, initial pH 7.0, and liquid volume 60 mL/250 mL. Under these conditions, the carotenoid yield of GH-1 is 7.24 mg/L, which indicated that gh-1 has a good performance in carotenoid production and could be used as a high-quality carotenoid strain resource. The carotenoids produced by Gh-1 strain have good free radical scavenging ability. This indicates that the pigment can be used as a potential antioxidant.