Protective Effect and Mechanism of Theanine on Alcohol-induced Injury of Human Hepatocytes HL7702

ObjectiveTo explore the protective effect and mechanism of theanine in resisting alcohol-induced damage to human liver HL7702 cells.

MethodSelect 6 alcohol concentration treatment groups in the range of 500-1 000 mmol, with a gradient of 100 mmol for initial treatment of HL7702 cells, select different concentrations of theanine to culture HL7702 cells, MTT method to determine the survival rate of the cells to determine the concentration of alcohol modeling And theanine concentration range. Cells treated with alcohol with different concentrations of theanine pre-protected in model concentration were measured to determine the cell survival rate; the contents of hydrogen peroxide (H₂O₂), malondialdehyde (MDA), superoxide dismutase activity (SOD), glutathione were measured with Glyphosate peroxidase (GSH-PX) content and total antioxidant capacity (T-AOC) level.

ResultsThe concentration of alcohol modeling was initially determined to be 800 mmol; HL7702 cells were not toxic when theanine concentration was below 2 000 g/mL, and the theanine concentration was adjusted to 200-800 μg/mL according to the actual situation; compared with the model group Compared, theanine significantly improved the survival rate of HL7702 in the alcohol injury model, and played a certain role in inhibiting the production of H₂O₂ and MDA in HL7702 cells, while the activities of SOD and GSH-PX increased, and the T-AOC capacity also increased.

ConclusionTheanine has been shown to protect human liver cells from oxidative damage induced by alcohol in different ways.The mechanism of action is that theanine protects normal hepatocytes from oxidative damage and oxidative stress response by inhibiting the lower of activity of key antioxidant enzymes and the production of intermediate in oxidation reaction under alcohol stimulation.