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LI Chun-yan, TANG Yuan-mou, LIANG Xiao-ling, LIU Wei, YAN Jun, GOU Xiao-jun. Determination of Oleuropein in Olive Leaves by HPLC-DAD[J]. Journal of Xihua University(Natural Science Edition), 2014, 33(2): 98-101. DOI: 10.3969/j.issn.1673-159X.2014.02.022
Citation: LI Chun-yan, TANG Yuan-mou, LIANG Xiao-ling, LIU Wei, YAN Jun, GOU Xiao-jun. Determination of Oleuropein in Olive Leaves by HPLC-DAD[J]. Journal of Xihua University(Natural Science Edition), 2014, 33(2): 98-101. DOI: 10.3969/j.issn.1673-159X.2014.02.022
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Determination of Oleuropein in Olive Leaves by HPLC-DAD

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  • The purpose of the study is to establish a method for qualitative and quantitative analysis oleuropein in olive leaves by high-performance liquid chromatography-diode array detector (HPLC-DAD). Reversed-phase HPLC is used to determine the contents of oleuropein in olive leaves by DAD with Akasil C18 column, using 23% acetonitrile as mobile phase under UV light of 230 nm at 35 ℃ and 1.0 mL/min.The Results indicate that the calibration curves of oleuropein have good linearity over the range from 0.0125 to 0.4 mg/mL with a correlation coeffient of 0.999 9, the yield of oleuropein in olive leaves is 0.39%.
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