Determination of Aflatoxin B1 in Rice by High Performance Liquid Chromatography with AflaTest Affinity Column

This study investigated the effect of high performance liquid chromatography (HPLC) with AflaTest affinity column on determination of aflatoxin B₁ in rice. Rice samples were extracted with methanol/water (80/20, V/V), followed by AflaTest affinity column for cleanup and then trifluoroacetic acid for derivatization, finally detected by the method of HPLC-FLD. The curve of calibration was well linear in the range of 0.01~2.5 ng, Y=60.355 x +0.054 3, R²=0.999 7. Furthermore, the detection limit was 0.1 μg/kg, and the recovery of the spiked AFB1 ranged from 89.26% to 99.45%, and the standard deviation ranged from 2.48% to 3.74%. Since the method had advantage of high selectivity, high sensitivity, and good detection effect, it was suitable for detecting the aflatoxin in massive grain or oil crops, which was favorable for demonstration and extension.