Effect of Tributyltin Chloride on Amylase and Lipase Activity in Ostrea Cucullata

To study the effect of tributyltin chloride on amylase and lipase activity in digestive gland, Ostrea cucullata were exposed to the tributyltin chloride of six different concentrations(0, 2, 5, 10, 50 and 100 ng·L^-1). After treatment of 1, 2, 3 and 4 d, the specific activities of amylase and lipase in the O. cucullata were analyzed by using enzyme analytical method respectively. It showed that (1) TBTCL had a marked inhibition effect on amylase activity in the digestive gland (P < 0.05) and the variation showed a parabola trend. There was the least inhibition effect at 10 ng·L^-1. It represented a dose-effect relationship. (2) In the different concentration of TBTCL, lipase activity was induced significantly (P < 0.05). There was higher induction at lower concentrations(2 ng·L^-1 and 5 ng·L^-1). The degree of induction by TBTCL decreased with the increase of TBTCL concentration. The phenomena above suggested that lipase activity was significantly related to the exposure dosage. Thus, it was deduced that the activity of amylase and lipase in O.cucullata show different variation patterns under different concentrations of TBTCL.