Optimization of Enzymolysis Conditions of Pea Protein Based on Response Surface Methodology

The purpose of this paper is to optimize enzymolysis conditions of pea protein by using response surface methodology, so as to set up an accurate enzymolysis prediction model. The enzymolysis conditions of pea protein with a water content of 30% by neutral protease were optimized to the scavenging ratio for DPPH and ^·OH by using response surface methodology. The optimal enzymolysis conditions were as follows: enzyme amount was 10.60%, temperature was 40.30 ℃, pH was 7.02, substrate concentration was 6.80%. Under this condition, the DPPH scavenging rate was 99.10% and the ^·OH scavenging rate was 97.60%. The model confirmed predicts accurately and reliably, and hydrolysates were high antioxidant. The model provides the valuable reference for related production and process of product development.