Abstract: This paper conducted a survey of autonomous mobile learning of college students in three universities, and comprehensively used various statistical methods to analyze the influence of mobile phone on the personalized autonomous mobile learning of college students from four dimensions of learning elements: personalized learning plan, personalized learning method, personalized learning content and personalized learning effect, taking into account the space-time dimensions of classroom and after-school. The study finds that college students' autonomous mobile learning by using mobile phone media coexists with their dependence on mobile phones; mobile media autonomous learning is an important way to realize personalized education for college students; individual characteristics have significant influence on personalized mobile learning of college students using mobile media. Finally, the paper puts forward some suggestions from the aspects of educational idea transformation, school system optimization, teachers' appropriate decentralization, students' autonomous learning, individual differences, etc., so as to give full play to the positive role of mobile media in personalized and autonomous mobile learning for college students and effectively realize personalized education for college students.